The lipid fluidity of rat liver membrane subfractions

Whetton, Anothony D., Houslay, Miles D., Dodd, Nicholas J. F. and Evans, William Howard 1983. The lipid fluidity of rat liver membrane subfractions. Biochemical Journal 214 (3) , pp. 851-854.

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Abstract

1. The lipid fluidity of three major rat liver plasma-membrane subfractions, as well as Golgi apparatus and endocytic fractions, was assessed with a fatty acid spin probe by using e.s.r. techniques. 2. The sinusoidal (blood-facing) plasma-membrane subfraction was the most fluid of the three plasma-membrane regions. Fractions originating from the bile-canalicular and contiguous (lateral) regions were most rigid. Endocytic fractions isolated (endosomes and diacytosomes) were of a similar fluidity to fractions originating from the sinusoidal plasma-membrane region. By far the most fluid fractions examined were derived from the Golgi-apparatus complex. 3. The three plasma-membrane subfractions each showed a different response to the bilayer-fluidizing effect of benzyl alcohol. 4. Arrhenius-type plots of the order parameter S and outer hyperfine splitting, 2T( parallel), identified lipid-phase separations in the plasma-membrane subfractions.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Medicine
Subjects:	R Medicine > R Medicine (General) R Medicine > RZ Other systems of medicine
Publisher:	Biochemical Society
ISSN:	0264-6021
Last Modified:	17 Mar 2021 02:56
URI:	https://orca.cardiff.ac.uk/id/eprint/66755

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