Fry, C. H., Griffiths, H. and Hall, Sarah K. 1993. Actions of extracellular chloride ion substitution on contractility of isolated ventricular myocardium. Cardiovascular Research 27 (5) , pp. 856-860. 10.1093/cvr/27.5.856 |
Abstract
Objective: Replacement of extracellular chloride ions by some other anions exerts a positive inotropic effect on isolated cardiac muscle preparations. The aim of this study was to determine whether the increase of force was mediated by an intracellular alkalosis. Methods: Isometric tension development was measured using guinea pig papillary muscles during replacement of superfusate CI−; with isethionate ions. Intracellular pH and L-type Ca2+ currents were measured in isolated myocytes using epifluorescence microscopy and a switch-clamp technique respectively. Results: Reduction of extracellular CI− increased tension, a phenomenon which was partially inhibited with the anion exchange blocker SITS. Force was also increased by reducing superfusate PCO2. Reduction of superfusate [CI−] and PCO2 both produced concentration dependent intracellular alkalosis. The relationship between the changes to tension and intracellular pH was different when either the superfusate [CI−] or PCO2 was reduced, less pronounced when the [CI−] was reduced. An increase of the L-type Ca2+ current was produced in low CI− solutions which could be attributed to the accompanying intracellular alkalosis. Conclusions: The positive inotropic effect of superfusate CI− removal can only partially be explained by intracellular alkalosis. Other, as yet unknown, changes must determine the remainder of the inotropic response.
Item Type: | Article |
---|---|
Date Type: | Publication |
Status: | Published |
Schools: | Biosciences |
Subjects: | Q Science > QR Microbiology |
Publisher: | Oxford University Press |
ISSN: | 0008-6363 |
Last Modified: | 04 Jun 2017 07:53 |
URI: | https://orca.cardiff.ac.uk/id/eprint/69546 |
Citation Data
Cited 5 times in Scopus. View in Scopus. Powered By Scopus® Data
Actions (repository staff only)
![]() |
Edit Item |