Characterization of plasmids in extensively drug-resistant acinetobacter strains isolated in India and Pakistan

Jones, Lim Stephen, Carvalho, Maria, Toleman, Mark Alexander Howard ORCID: https://orcid.org/0000-0002-9497-0512, White, Peter, Connor, Thomas Richard ORCID: https://orcid.org/0000-0003-2394-6504, Mushtaq, Ammara, Weeks, Janis Lesley, Kumarasamy, Karthikeyan K., Raven, Katherine E., Török, M. Estée, Peacock, Sharon J., Howe, Robin A. and Walsh, Timothy Rutland ORCID: https://orcid.org/0000-0003-4315-4096 2015. Characterization of plasmids in extensively drug-resistant acinetobacter strains isolated in India and Pakistan. Antimicrobial Agents and Chemotherapy 59 (2) , pp. 923-929. 10.1128/AAC.03242-14

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Abstract

The blaNDM-1 gene is associated with extensive drug resistance in Gram-negative bacteria. This probably spread to Enterobacteriaceae from Acinetobacter spp., and we characterized plasmids associated with blaNDM-1 in Acinetobacter spp. to gain insight into their role in this dissemination. Four clinical NDM-1-producing Acinetobacter species strains from India and Pakistan were investigated. A plasmid harboring blaNDM-1, pNDM-40-1, was characterized by whole-genome sequencing of Acinetobacter bereziniae CHI-40-1 and comparison with related plasmids. The presence of similar plasmids in strains from Pakistan was sought by PCR and sequencing of amplicons. Conjugation frequency was tested and stability of pNDM-40-1 investigated by real-time PCR of isolates passaged with and without antimicrobial selection pressure. A. bereziniae and Acinetobacter haemolyticus strains contained plasmids similar to the pNDM-BJ01-like plasmids identified in Acinetobacter spp. in China. The backbone of pNDM-40-1 was almost identical to that of pNDM-BJ01-like plasmids, but the transposon harboring blaNDM-1, Tn125, contained two short deletions. Escherichia coli and Acinetobacter pittii transconjugants were readily obtained. Transconjugants retained pNDM-40-1 after a 14-day passage experiment, although stability was greater with meropenem selection. Fragments of pNDM-BJ01-like plasmid backbones are found near blaNDM-1 in some genetic contexts from Enterobacteriaceae, suggesting that cross-genus transfer has occurred. pNDM-BJ01-like plasmids have been described in isolates originating from a wide geographical region in southern Asia. In vitro data on plasmid transfer and stability suggest that these plasmids could have contributed to the spread of blaNDM-1 into Enterobacteriaceae.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Biosciences Medicine Systems Immunity Research Institute (SIURI)
Subjects:	R Medicine > R Medicine (General)
Publisher:	American Society for Microbiology
ISSN:	0066-4804
Date of Acceptance:	16 November 2014
Last Modified:	28 Oct 2022 08:29
URI:	https://orca.cardiff.ac.uk/id/eprint/70977

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