Jiang, Wen Guo ![]() ![]() |
Abstract
HGF/SF (0.5-100 ng/ml) induced rapid membrane ruffling, formation of microspikes, and increased motility of HT115 cells within 5 min of addition. These effects were not accompanied by any change in cytosolic free Ca2+ concentration. However, ATP (0.5-10 mM) induced a transient rise in cytosolic free Ca2+ concentration in HT115 cells from a resting concentration of 100 nM to a peak of 400 nM before returning to baseline within 3 min. The addition of ATP to cells treated with HGF/SF inhibited both membrane ruffling and cell movement. The effect of ATP was attributed to the transient rise in cytosolic free Ca2+ concentration, because cytosolic BAPTA, which prevented the rise in cytosolic free Ca2+ concentration, also abolished the inhibitory effect of ATP. Raising cytosolic free Ca2+ concentration with ionomycin and ADP also inhibited membrane ruffling. It was thus concluded that transiently raised cytosolic free Ca2+ concentration inhibited HGF/SF-induced membrane ruffling of HT115 cells.
Item Type: | Article |
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Date Type: | Publication |
Status: | Published |
Schools: | Medicine |
Subjects: | R Medicine > R Medicine (General) |
Publisher: | Elsevier |
ISSN: | 0014-4827 |
Last Modified: | 28 Oct 2022 09:00 |
URI: | https://orca.cardiff.ac.uk/id/eprint/72936 |
Citation Data
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