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The cloning and overexpression of escherichia-coli acyl carrier protein

Jones, Lesley, Kille, Peter, Dancer, J. E. and Harwood, J. L. 1993. The cloning and overexpression of escherichia-coli acyl carrier protein. Grasas Y Aceites 44 (2) , pp. 116-117.

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Acyl carrier protein (ACP) from E. coli was cloned using degenerate PCR. The resulting clone was sequenced and translated to give a product which had the known amino acid sequence of E. coli ACP. The insert obtained was cloned into various expression systems, but no overexpression was obtained and sequencing of the insert revealed the presence of mutations.

Item Type: Article
Date Type: Publication
Status: Published
Schools: MRC Centre for Neuropsychiatric Genetics and Genomics (CNGG)
Subjects: R Medicine > R Medicine (General)
Publisher: Instituto de la Grasa Y Sus Derivados / Consejo Superior de Investigaciones Cientificas
ISSN: 0017-3495
Last Modified: 21 Jun 2019 19:41

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