Abo1, a conserved bromodomain AAA-ATPase, maintains global nucleosome occupancy and organisation

Gal, Csenge, Murton, Heather E., Subramanian, Lakxmi, Whale, Alex J., Moore, Karen M., Paszkiewicz, Konrad, Codlin, Sandra, Bahler, Jurg, Creamer, Kevin M., Partridge, Janet F., Allshire, Robin C., Kent, Nicholas A. ORCID: https://orcid.org/0000-0002-4114-1307 and Whitehall, Simon K. 2016. Abo1, a conserved bromodomain AAA-ATPase, maintains global nucleosome occupancy and organisation. EMBO reports 17 (1) , pp. 79-93. 10.15252/embr.201540476

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Abstract

Maintenance of the correct level and organisation of nucleosomes is crucial for genome function. Here, we uncover a role for a conserved bromodomain AAA‐ATPase, Abo1, in the maintenance of nucleosome architecture in fission yeast. Cells lacking abo1+ experience both a reduction and mis‐positioning of nucleosomes at transcribed sequences in addition to increased intragenic transcription, phenotypes that are hallmarks of defective chromatin re‐establishment behind RNA polymerase II. Abo1 is recruited to gene sequences and associates with histone H3 and the histone chaperone FACT. Furthermore, the distribution of Abo1 on chromatin is disturbed by impaired FACT function. The role of Abo1 extends to some promoters and also to silent heterochromatin. Abo1 is recruited to pericentromeric heterochromatin independently of the HP1 ortholog, Swi6, where it enforces proper nucleosome occupancy. Consequently, loss of Abo1 alleviates silencing and causes elevated chromosome mis‐segregation. We suggest that Abo1 provides a histone chaperone function that maintains nucleosome architecture genome‐wide

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Biosciences
Subjects:	Q Science > QH Natural history > QH426 Genetics
Additional Information:	This is an open access article under the terms of the Creative Commons Attribution 4.0 License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
Publisher:	EMBO Press
ISSN:	1469-221X
Date of First Compliant Deposit:	30 March 2016
Date of Acceptance:	26 October 2015
Last Modified:	05 May 2023 21:54
URI:	https://orca.cardiff.ac.uk/id/eprint/82919

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