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Neutron and X-ray scattering by ox corneal stroma differentially loaded with bound anions

Regini, Justyn Wiktor, Timmins, P. A., Elliott, Gerald and Hodson, Stuart Arthur 2003. Neutron and X-ray scattering by ox corneal stroma differentially loaded with bound anions. Biochimica et biophysica acta (BBA) - General subjects 1620 (1-3) , pp. 54-58. 10.1016/S0304-4165(02)00506-8

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Ox corneas at near physiological hydration were subjected to two variables: the amount of chloride ions bound to them and exposure of various mixtures of H2O/D2O as solvent. The preparations were then exposed to a neutron beam and the contrast match points, at which the collagen fibrils of the corneal stroma most nearly matched the scattering density of the various H2O/D2O mixtures, were measured. In both cases of high and low bound chloride, the contrast match points of the collagen fibril were equal, indicating that there were no significant changes in the water of electrostriction at the fibril surface when chloride ions bind to the stroma. The data suggest that the ligands which bind anions to corneal stroma are not located at the collagen fibril surface. When the chloride binding ligands were extracted from the corneal stroma there were significant changes in the structure of the fibrils. We suggest that the chloride binding ligands may be located within the collagen fibril.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Optometry and Vision Sciences
Uncontrolled Keywords: Cornea ; Neutron scattering ; Anion binding ; Transparency ; Collagen fibril
Publisher: Elsevier
ISSN: 03044165
Last Modified: 04 Jun 2017 01:36

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