Virulence and pathogenicity of Candida albicans is enhanced in biofilms containing oral bacteria

Cavalcanti, Yuri Wanderley, Morse, Daniel James ORCID: https://orcid.org/0000-0002-0104-4940, da Silva, Wander José, Del-Bel-Cury, Altair Antoninha, Wei, Xiaoqing ORCID: https://orcid.org/0000-0002-6274-8503, Wilson, Melanie ORCID: https://orcid.org/0000-0002-8834-8268, Milward, Paul, Lewis, Michael ORCID: https://orcid.org/0000-0003-1917-0651, Bradshaw, David and Williams, David Wynne ORCID: https://orcid.org/0000-0002-7351-5131 2015. Virulence and pathogenicity of Candida albicans is enhanced in biofilms containing oral bacteria. Biofouling 31 (1) , pp. 27-38. 10.1080/08927014.2014.996143

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Abstract

This study examined the influence of bacteria on the virulence and pathogenicity of candidal biofilms. Mature biofilms (Candida albicans-only, bacteria-only, C. albicans with bacteria) were generated on acrylic and either analysed directly, or used to infect a reconstituted human oral epithelium (RHOE). Analyses included Candida hyphae enumeration and assessment of Candida virulence gene expression. Lactate dehydrogenase (LDH) activity and Candida tissue invasion following biofilm infection of the RHOE were also measured. Candida hyphae were more prevalent (p < 0.05) in acrylic biofilms also containing bacteria, with genes encoding secreted aspartyl-proteinases (SAP4/SAP6) and hyphal-wall protein (HWP1) up-regulated (p < 0.05). Candida adhesin genes (ALS3/EPA1), SAP6 and HWP1 were up-regulated in mixed-species biofilm infections of RHOE. Multi-species infections exhibited higher hyphal proportions (p < 0.05), up-regulation of IL-18, higher LDH activity and tissue invasion. As the presence of bacteria in acrylic biofilms promoted Candida virulence, consideration should be given to the bacterial component when managing denture biofilm associated candidoses.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Dentistry
Subjects:	R Medicine > RK Dentistry
Uncontrolled Keywords:	biofilm, Candida albicans, candidosis, co-infection, RHOE, virulence
Publisher:	Taylor & Francis
ISSN:	0892-7014
Date of Acceptance:	2 December 2014
Last Modified:	06 Dec 2022 02:11
URI:	https://orca.cardiff.ac.uk/id/eprint/85292

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