A highly sensitive HPLC method for determination of nanomolar concentrations of dipicolinic acid, a characteristic constituent of bacterial endospores

Fichtel, Jörg, Köster, Jürgen, Scholz-Böttcher, Barbara, Sass, Henrik ORCID: https://orcid.org/0000-0001-8740-4224 and RullKötter, Jürgen 2007. A highly sensitive HPLC method for determination of nanomolar concentrations of dipicolinic acid, a characteristic constituent of bacterial endospores. Journal of Microbiological Methods 70 (2) , pp. 319-327. 10.1016/j.mimet.2007.05.008

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Abstract

A high-performance liquid chromatographic method with indirect fluorescence detection has been developed for quantification of dipicolinic acid, a major constituent of bacterial endospores. After separation on a reversed-phase column, a post-column reagent of sodium acetate at 1 mol l− 1 with 50 μmol l− 1 terbium chloride was added for complexation of dipicolinic acid. Terbium monodipicolinate complexes formed were quantified by measuring the fluorescence emission maximum at 548 nm after excitation with UV light at 270 nm wavelength. Parameters of post-column complexation were optimized to achieve a detection limit of 0.5 nmol DPA l− 1, corresponding to about 103 Desulfosporosinus orientis endospores per ml. The method was applied to the analysis of spore contamination in tuna and for estimating the endospore numbers in marine sediments.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Earth and Environmental Sciences
Subjects:	Q Science > QD Chemistry Q Science > QR Microbiology
Uncontrolled Keywords:	Dipicolinic acid ; Post-column complexation ; HPLC ; Terbium dipicolinate fluorescence ; Endospores; Quantification.
ISSN:	01677012
Last Modified:	17 Oct 2022 10:34
URI:	https://orca.cardiff.ac.uk/id/eprint/8557

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