Effect of junctional adhesion molecule-2 expression on cell growth, invasion and migration in human colorectal cancer

Zhao, Huishan, Yu, Hefen, Martin, Tracey Amanda ORCID: https://orcid.org/0000-0003-2690-4908, Zhang, Yuxiang, Chen, Gang and Jiang, Wen Guo ORCID: https://orcid.org/0000-0002-3283-1111 2016. Effect of junctional adhesion molecule-2 expression on cell growth, invasion and migration in human colorectal cancer. International Journal of Oncology 48 (3) , pp. 929-936. 10.3892/ijo.2016.3340

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Abstract

The junctional adhesion molecule (JAMs) family belongs to the immunoglobulin subfamily involved in the formation of tight junctions (TJ) in both endothelial and epithelial cells. Aberrant expression of JAM-2 is associated with cancer progression but little work has been carried out in discovering how this affects changes in cell behaviour. The present study aimed to examine the expression of JAM-2 in human colon cancer specimens and cell lines and its role in the development of colon cancer. JAM-2 expression in human colon cancer specimens (normal, n=75; cancer, n=94) and cell lines was analysed using quantitative real-time PCR and conventional RT-PCR. Colon cancer cells were stably transfected with a mammalian expression vector to overexpress JAM-2-Flag. The effect on growth, adhesion and migration following overexpression of JAM-2 was then investigated using in vitro models. TJ function was assessed using a trans-epithelial resistance assay (TER, with an EVOM voltammeter). JAM-2 was lowly expressed in colon cancer cells such as RKO, HT115. JAM-2 overexpression in RKO cells (RKO-JAM-2) and HT115 cells (HT115-JAM-2) showed retarded adhesion (P<0.05). An in vivo tumour model showed that RKO-JAM-2 had significantly reduced growth (P<0.05), invasion (P<0.05) and migration (P<0.05) as well as in HT115-JAM-2, except on proliferation and migration. Expression of JAM-2 resulted in a significant increase in TER and decrease in permeability of polarized monolayers (P<0.05). Further analysis of JAM-2 transcript levels against clinical aspects demonstrated that the decreasing JAM-2 expression correlated to disease progression, metastasis and poor survival. Taken together, JAM-2 may function as a putative tumour suppressor in the progression and metastasis of colorectal cancer

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Medicine
Subjects:	R Medicine > RC Internal medicine > RC0254 Neoplasms. Tumors. Oncology (including Cancer)
Additional Information:	Published online on: Friday, January 15, 2016 This is an open access article distributed under the terms of Creative Commons Attribution License
Publisher:	Spandidos Publications
ISSN:	1019-6439
Date of First Compliant Deposit:	30 March 2016
Date of Acceptance:	5 November 2015
Last Modified:	06 May 2023 04:21
URI:	https://orca.cardiff.ac.uk/id/eprint/86235

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