Cholecystokinin-58 and cholecystokinin-8 exhibit similar actions on calcium signaling, zymogen secretion, and cell fate in murine pancreatic acinar cells

Criddle, D. N, Booth, D. M, Mukherjee, R., McLaughlin, E., Green, G. M., Sutton, R., Petersen, Ole Holger ORCID: https://orcid.org/0000-0002-6998-0380 and Reeve, J. R. 2009. Cholecystokinin-58 and cholecystokinin-8 exhibit similar actions on calcium signaling, zymogen secretion, and cell fate in murine pancreatic acinar cells. American Journal of Physiology-Gastrointestinal and Liver Physiology 297 (6) , G1085-G1092. 10.1152/ajpgi.00119.2009

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Abstract

The gastrointestinal hormone CCK exists in various molecular forms, with differences in bioactivity between the well-characterized CCK-8 and larger CCK-58 previously reported. We have compared the effects of these peptides on cytosolic calcium concentration ([Ca2+]c), mitochondrial metabolism, enzyme secretion, and cell fate in murine isolated pancreatic acinar cells using fluorescence confocal microscopy and patch-clamp electrophysiology. CCK-58 (1–10 pM) induced transient, oscillatory increases of [Ca2+]c, which showed apical to basolateral progression and were associated with a rise of mitochondrial NAD(P)H. CCK-58 (10 pM) induced zymogen exocytosis in isolated cells and amylase secretion from isolated cells and whole tissues. Hyperstimulation with supraphysiological CCK-58 (5 nM) induced a single large increase of [Ca2+]c that declined to a plateau, which remained above the basal level 20 min after application and was dependent on external Ca2+ entry. In cells dispersed from the same tissues, CCK-8 induced similar patterns of responses to those of CCK-58, with oscillatory increases of [Ca2+]c at lower (pM) concentrations and sustained responses at 5 nM. CCK-58 and CCK-8 exhibited similar profiles of action on cell death, with increases in necrosis at high CCK-58 and CCK-8 (10 nM) that were not significantly different between peptides. The present experiments indicate that CCK-8 and CCK-58 have essentially identical actions on the acinar cell at high and low agonist concentrations, suggesting an action via the same receptor and that the differences observed in an intact rat model may result from indirect effects of the peptides. Our data strengthen the argument that CCK-58 is an important physiological form of this gastrointestinal hormone.

Item Type:	Article
Status:	Published
Schools:	Biosciences Systems Immunity Research Institute (SIURI)
Subjects:	Q Science > QM Human anatomy Q Science > QP Physiology
Uncontrolled Keywords:	Mitochondria; necrosis; apoptosis
Publisher:	American Society for Biochemistry and Molecular Biology
ISSN:	0193-1857
Last Modified:	17 Oct 2022 10:36
URI:	https://orca.cardiff.ac.uk/id/eprint/8711

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