Development and evaluation of a calibrator material for nucleic acid-based assays for diagnosing Aspergillosis

Lyon, G. M., Abdul-Ali, D., Loeffler, J., White, P., Wickes, B., Herrera, M., Alexander, B., Baden, L., Clancy, C., Denning, D., Nguyen, M., Sugrue, M., Wheat, L., Wingard, J., Donnelly, J., Barnes, Rosemary Ann, Patterson, T. and Caliendo, A. 2013. Development and evaluation of a calibrator material for nucleic acid-based assays for diagnosing Aspergillosis. Journal of Clinical Microbiology 51 (7) , pp. 2403-2405. 10.1128/JCM.00744-13

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Abstract

Twelve laboratories evaluated candidate material for an Aspergillus DNA calibrator. The DNA material was quantified using limiting-dilution analysis; the mean concentration was determined to be 1.73-1010 units/ml. The calibrator can be used to standardize aspergillosis diagnostic assays which detect and/or quantify nucleic acid.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Medicine
Subjects:	R Medicine > R Medicine (General) R Medicine > RZ Other systems of medicine
Uncontrolled Keywords:	Aspergillosis; Aspergillus; DNA, Fungal; Humans; Microbiological Techniques; Molecular Diagnostic Techniques; Reference Standards
Publisher:	American Society for Microbiology
ISSN:	0095-1137
Date of Acceptance:	18 April 2013
Last Modified:	04 Jun 2017 08:53
URI:	https://orca.cardiff.ac.uk/id/eprint/87258

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