Hyperspectral volumetric coherent anti-Stokes Raman scattering microscopy: quantitative volume determination and NaCl as non-resonant standard

Karuna, Arnica, Masia, Francesco ORCID: https://orcid.org/0000-0003-4958-410X, Borri, Paola ORCID: https://orcid.org/0000-0002-7873-3314 and Langbein, Wolfgang Werner ORCID: https://orcid.org/0000-0001-9786-1023 2016. Hyperspectral volumetric coherent anti-Stokes Raman scattering microscopy: quantitative volume determination and NaCl as non-resonant standard. Journal of Raman Spectroscopy 47 (9) , pp. 1167-1173. 10.1002/jrs.4876

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Abstract

In this work, we demonstrate quantitative volume determination of chemical components in three dimensions using hyperspectral coherent anti-Stokes Raman scattering microscopy, phase-corrected Kramers–Kronig retrieval of the coherent anti-Stokes Raman scattering susceptibility and factorization into concentration of chemical components. We investigate the influence of the refractive index contrast between water and polymer beads (polystyrene and polymethylmethacrylate), showing that it leads mainly to concentration errors, while the spectral error is less affected. The volume of polystyrene beads of sizes from 200 nm to 3 μm is determined with 10% relative error and 1% absolute error in the region of interest. We furthermore establish the use of sodium chloride as non-resonant reference material free of Raman-active vibrational resonances.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Biosciences Physics and Astronomy
Subjects:	Q Science > QC Physics
Publisher:	Wiley-Blackwell
ISSN:	0377-0486
Funders:	EPSRC, BBSRC
Date of First Compliant Deposit:	30 March 2016
Date of Acceptance:	11 December 2015
Last Modified:	05 May 2023 14:40
URI:	https://orca.cardiff.ac.uk/id/eprint/87413

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