Cadmium stress alters gene expression of DNA mismatch repair related genes in Arabidopsis seedlings

Liu, W., Yang, Yuesuo, Francis, Dennis, Rogers, Hilary Joan ORCID: https://orcid.org/0000-0003-3830-5857 and Zhang, Q. 2008. Cadmium stress alters gene expression of DNA mismatch repair related genes in Arabidopsis seedlings. Chemosphere 73 (7) , pp. 1138-1144. 10.1016/j.chemosphere.2008.07.020

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Abstract

Cadmium (Cd) is a non essential element, and is a widespread environmental pollutant. Exposure to Cd can result in a variety of adverse health effects in plant and humans. In the current study, Arabidopsis seedlings were used as a bio-indicator of Cd pollution. Seedlings were grown on MS media containing 0–6.0 mg L−1 Cd for 18 days, and the gene expression patterns were used to link increased Cd exposure with progressive biological effects. Reduction of total soluble protein content in shoots of the Arabidopsis seedlings occurred with increase in Cd concentrations. For the gene expression patterns, seven genes known to be involved in cell division and DNA mismatch repair (MMR) system were investigated by semi-quantitative RT-PCR, and normalized using 18 S rRNA gene expression. Expression of the proliferating cell nuclear antigen 2 (atPCNA 2), MutS 3 homolog (atMSH 3) and MutL1 homolog (atMLH1) genes in shoots of Arabidopsis was strongly induced by exposure to 0.75 mg L−1 Cd, but were repressed by other Cd concentrations whereas exposure to 0.75–6 mg L−1 of Cd resulted in a decreased expression of atPCNA1, atMSH 2, 6 and 7 genes independently of any observable biological effects, including survival, fresh weight and chlorophyll level of shoots. This work demonstrated that specific gene expression changes could serve as useful molecular biomarkers indicative of Cd exposure and related biological effects.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Biosciences Earth and Environmental Sciences
Subjects:	G Geography. Anthropology. Recreation > GE Environmental Sciences Q Science > QK Botany T Technology > TA Engineering (General). Civil engineering (General) T Technology > TD Environmental technology. Sanitary engineering
Uncontrolled Keywords:	Cadmium; Gene expression; DNA mismatch repair genes; PCNA genes; Biomarker
Publisher:	Elsevier
ISSN:	0045-6535
Last Modified:	17 Oct 2022 10:36
URI:	https://orca.cardiff.ac.uk/id/eprint/8749

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