The association between WAVE1 and -3 and the ARP2/3 complex in PC 3 cells

Weeks, Hoi, Sanders, Andrew ORCID: https://orcid.org/0000-0002-7997-5286, Kynaston, Howard ORCID: https://orcid.org/0000-0003-1902-9930 and Jiang, Wen ORCID: https://orcid.org/0000-0002-3283-1111 2016. The association between WAVE1 and -3 and the ARP2/3 complex in PC 3 cells. Anticancer Research 36 (3) , pp. 1135-1142.

Full text not available from this repository.

Abstract

Background: Actin polymerisation is stimulated by the actin-related protein (ARP) 2/3 complex and drives cell migration. This complex is activated by Wiskott-Aldrich syndrome protein family (WASP) verprolin homologous protein (WAVE) proteins. WAVE1 and -3 have been implicated in the aggressiveness of metastatic prostate cancer cells. Materials and Methods: Cell growth, motility and invasion were analyzed in WAVE1- and WAVE3-knockdown PC-3 cells along with the ARP2/3 inhibitor, CK-0944636. Confocal microscopy was adopted to examine protein co-localisation. Immunoprecipitation approaches were used to determine protein tyrosine phosphorylation. Results: Cell growth suppression was observed with WAVE3 knockdown and ARP2/3 inhibition. Reduced cell invasion effects observed with WAVE1 knockdown appeared to be rescued by ARP2/3 inhibition. WAVE1 and WAVE3 and ARP2 co-localisation was lost in PC-3 WAVE-knockdown cells, while increased ARP2 tyrosine phosphorylation was observed with WAVE3 knockdown. Conclusion: These results implicate a contributory role of WAVE1 and -3 to the metastatic phenotype of PC-3 cells through their interaction with the ARP2/3 complex.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Medicine
Subjects:	R Medicine > RC Internal medicine > RC0254 Neoplasms. Tumors. Oncology (including Cancer)
Publisher:	International Institute of Anticancer Research
ISSN:	1791-7530
Date of Acceptance:	22 February 2016
Last Modified:	07 Nov 2024 22:16
URI:	https://orca.cardiff.ac.uk/id/eprint/88361

Citation Data

Cited 1 time in Scopus. View in Scopus. Powered By Scopus® Data

Actions (repository staff only)

Edit Item