Hepatocyte growth factor/scatter factor decreases the expression of occludin and transendothelial resistance (TER) and increases paracellular permeability in human vascular endothelial cells.

Jiang, Wen Guo ORCID: https://orcid.org/0000-0002-3283-1111, Martin, Tracey Amanda ORCID: https://orcid.org/0000-0003-2690-4908, Matsumoto, Kei, Nakamur, T. and Mansel, Robert Edward ORCID: https://orcid.org/0000-0002-8051-0726 1999. Hepatocyte growth factor/scatter factor decreases the expression of occludin and transendothelial resistance (TER) and increases paracellular permeability in human vascular endothelial cells. Journal of Cellular Physiology 181 (2) , pp. 219-329. 10.1002/(SICI)1097-4652(199911)181:2<319::AID-JCP14>3.0.CO;2-S

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Abstract

Hepatocyte growth factor/scatter factor (HGF/SF), a multi function cytokine has been shown to regulate the expression of cell adhesion molecules in endothelial cells. In the current study, we examined the effects of HGF/SF on the function of tight junctions and the expression of occludin in these cells. Stimulation of human vascular endothelial cells with HGF/SF resulted in a concentration dependent increase in the paracellular permeability as measured using fluorescence labelled dextran but a decrease in the transendothelial cell resistance (TER) of the endothelial cells. Western blotting revealed that HGF/SF decreased the level of occludin in the cells, a primary tight junction forming protein. Immunofluorescence study indicated that shortly after treatment with HGF/SF there was a disturbance of the distribution of occludin and then a reduction in the staining of the molecule. It is concluded that HGF/SF decreases the expression of occludin, resulting in the functional change of tight junction.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Medicine
Subjects:	R Medicine > R Medicine (General)
Publisher:	Wiley
ISSN:	0021-9541
Last Modified:	01 Nov 2022 09:40
URI:	https://orca.cardiff.ac.uk/id/eprint/88802

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