Ca2+ dynamics in oocytes from naturally-aged mice

Haverfield, Jenna, Nakagawa, Shoma, Love, Daniel, Tsichlaki, Elina, Nomikos, Michail, Lai, Francis Anthony ORCID: https://orcid.org/0000-0003-2852-8547, Swann, Karl ORCID: https://orcid.org/0000-0002-4355-1449 and FitzHarris, Greg 2016. Ca2+ dynamics in oocytes from naturally-aged mice. Scientific Reports 6 , 19357. 10.1038/srep19357

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Abstract

The ability of human metaphase-II arrested eggs to activate following fertilisation declines with advancing maternal age. Egg activation is triggered by repetitive increases in intracellular Ca2+ concentration ([Ca2+]i) in the ooplasm as a result of sperm-egg fusion. We therefore hypothesised that eggs from older females feature a reduced ability to mount appropriate Ca2+ responses at fertilisation. To test this hypothesis we performed the first examination of Ca2+ dynamics in eggs from young and naturally-aged mice. Strikingly, we find that Ca2+ stores and resting [Ca2+]i are unchanged with age. Although eggs from aged mice feature a reduced ability to replenish intracellular Ca2+ stores following depletion, this difference had no effect on the duration, number, or amplitude of Ca2+ oscillations following intracytoplasmic sperm injection or expression of phospholipase C zeta. In contrast, we describe a substantial reduction in the frequency and duration of oscillations in aged eggs upon parthenogenetic activation with SrCl2. We conclude that the ability to mount and respond to an appropriate Ca2+ signal at fertilisation is largely unchanged by advancing maternal age, but subtle changes in Ca2+ handling occur that may have more substantial impacts upon commonly used means of parthenogenetic activation.

Item Type:	Article
Date Type:	Published Online
Status:	Published
Schools:	Biosciences Medicine
Publisher:	Nature Publishing Group
ISSN:	2045-2322
Date of First Compliant Deposit:	12 April 2016
Date of Acceptance:	11 December 2015
Last Modified:	05 May 2023 00:26
URI:	https://orca.cardiff.ac.uk/id/eprint/89090

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