Flow cytometric monitoring of rhodamine 123 and a cyanine dye uptake by yeast during cider fermentation

Lloyd, David ORCID: https://orcid.org/0000-0002-5656-0571, Moran, Colm A., Suller, Marc T. E., Dinsdale, M. Gwenda and Hayes, Anthony Joseph 1996. Flow cytometric monitoring of rhodamine 123 and a cyanine dye uptake by yeast during cider fermentation. Journal of the Institute of Brewing 102 (4) , pp. 251-259. 10.1002/j.2050-0416.1996.tb00910.x

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Abstract

Flow cytometry of rhodamine 123- or cyanine-stained cider yeast shows that the capacity for mitochondrial uptake of these cationic dyes is lost early in the fermentation. Survival of prolonged anaerobiosis (for at least 22 days) at high ethanol concentrations (at least 11% v/v) during cider fermentation does not require the maintenance of a measurable inner mitochondrial transmembrane electrochemical potential. Confocal laser scanning microscopy of yeasts after exposure to either of the cationic dyes confirms the lack of mitochondrial development and inability of the fermentative organisms to take up the fluorophores. Aeration of samples taken from the fermentation vessels restores the ultrastructure and the dye uptake capacity of the yeasts. This indicates that the changes are reversible, and that the organisms have retained their viability.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Biosciences
Publisher:	Wiley-Blackwell
ISSN:	0046-9750
Last Modified:	01 Nov 2022 09:51
URI:	https://orca.cardiff.ac.uk/id/eprint/89441

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