Aldahlawi, Nada Hussein, Hayes, Sally  ORCID: https://orcid.org/0000-0001-8550-0108, O'Brart, David P. S., Akhbanbetova, Alina, Littlechild, Stacy Lee and Meek, Keith Michael Andrew ORCID: https://orcid.org/0000-0002-9948-7538
2016.
Enzymatic resistance of corneas crosslinked using riboflavin in conjunction with low energy, high energy, and pulsed UVA irradiation modes.
Investigative Ophthalmology & Visual Science
57
(4)
, pp. 1547-1552.
10.1167/iovs.15-18769
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Abstract
Purpose: To investigate the effect of various riboflavin/ultraviolet light (UVA) crosslinking (CXL) protocols on corneal enzymatic resistance. Methods: A total of 66 enucleated porcine eyes, with the corneal epithelium removed, were divided into 6 groups. Group 1 remained untreated. Groups 2 to 6 received riboflavin/dextran for 30 minutes. Group 3 underwent standard CXL (SCXL) with 3 mW/cm2 UVA for 30 minutes (total energy dose 5.4 J/cm2). Groups 4 and 5 underwent high intensity CXL (HCXL) using 30 mW/cm2 UVA for 3 minutes (5.4 J/cm2) and 30 mW/cm2 for 4 minutes (7.2 J/cm2), respectively. Group 6 was exposed to 8 minutes of 30 mW/cm2 UVA in a 10-second on/10-second off pulsed-radiation mode (p-HCXL; 7.2 J/cm2). A central 8-mm disk from each cornea was submerged in pepsin digest solution at 23°C and measured daily. After 13 days, the dry weight was recorded from 5 samples in each group. Results: The CXL-treated corneas took longer to digest than nonirradiated corneas (P < 0.0001). Differences in digestion time also were observed between CXL groups, such that, HCXL (5.4 J/cm2) < SCXL (5.4 J/cm2) < HCXL (7.2 J/cm2) < p-HCXL (7.2 J/cm2; P < 0.0001). The dry weight of the SCXL (5.4 J/cm2) group was higher than the HCXL (5.4 and 7.2 J/cm2; P < 0.001) and p-HCXL 7.2 J/cm2 (P <0.05) groups. No difference was detected between the HCXL and p-HCXL 7.2 J/cm2 groups. Conclusions: The intensity and distribution of the crosslinks formed within the cornea vary with different UVA protocols. The precise location and amount of crosslinking needed to prevent disease progression is unknown.
| Item Type: | Article |
|---|---|
| Date Type: | Publication |
| Status: | Published |
| Schools: | Schools > Optometry and Vision Sciences |
| Subjects: | R Medicine > RE Ophthalmology |
| Publisher: | Association for Research in Vision and Ophthalmology |
| ISSN: | 1552-5783 |
| Funders: | Medical Research Council |
| Date of First Compliant Deposit: | 26 April 2016 |
| Date of Acceptance: | 20 January 2016 |
| Last Modified: | 05 May 2023 23:03 |
| URI: | https://orca.cardiff.ac.uk/id/eprint/90065 |
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