Potential implications of interleukin-7 in chronic wound healing

Bartlett, Annie, Sanders, Andrew James ORCID: https://orcid.org/0000-0002-7997-5286, Ruge, Fiona, Harding, Keith Gordon and Jiang, Wen Guo ORCID: https://orcid.org/0000-0002-3283-1111 2016. Potential implications of interleukin-7 in chronic wound healing. Experimental and Therapeutic Medicine 12 (1) , pp. 33-40. 10.3892/etm.2016.3263

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Abstract

Methods of identifying chronic wounds that will heal in a timely, coordinated fashion and those that will not, together with novel therapeutic strategies, are vital for progression in the field of wound healing. Interleukin (IL)‑7 has been associated with various biological and pathological processes. The present study explored the potential role of IL‑7 in wound healing. IL-7 expression levels were examined in a clinical cohort of chronic wounds using reverse transcription-quantitative polymerase chain reaction and immunohistochemical staining analysis. The impact of recombinant human IL‑7 (rhIL-7) on the growth and migrational rates of HaCaT keratinocyte cells was subsequently examined using in vitro growth and electric cell‑substrate impedance sensing functional assays. The mRNA expression levels of IL‑7 were increased in the healed chronic wound tissue samples, compared with non‑healed chronic wound tissue samples, although the difference was not statistically significant. Similarly, immunohistochemical analysis revealed a greater staining intensity of IL‑7 in the healed chronic wound tissue sections compared with the non‑healed tissue sections. Treatment with rhIL‑7 did not affect HaCaT cell growth rates, but was shown to enhance cell migration, an effect that could be further enhanced through the addition of inhibitors of neuronal Wiskott‑Aldrich syndrome protein and protein kinase B. The data of the present study suggest that the expression levels of IL‑7 may be increased in healing chronic wounds, and thus IL‑7 may have a role in this process, potentially through its effects on the cellular migration of keratinocytes.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Medicine
Subjects:	R Medicine > R Medicine (General)
Publisher:	Spandidos
ISSN:	1792-0981
Date of First Compliant Deposit:	18 May 2016
Date of Acceptance:	8 December 2015
Last Modified:	23 May 2023 18:55
URI:	https://orca.cardiff.ac.uk/id/eprint/90994

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