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Peptones from diverse sources: pivotal determinants of bacterial growth dynamics

Gray, Victoria L., Müller, Carsten Theodor, Watkins, I. D. and Lloyd, David 2008. Peptones from diverse sources: pivotal determinants of bacterial growth dynamics. Journal of Applied Microbiology 104 (2) , pp. 554-565. 10.1111/j.1365-2672.2007.03577.x

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Aims:  In view of the major problems encountered by microbiologists in obtaining reproducible data on growth dynamics in complex media, we studied the effects of different peptones made from different biological sources and produced by numerous manufacturers. Methods and Results:  Peptones (including casein, gelatin, meat, soy and yeast) were assessed as a constituent of the pre-enrichment broth buffered peptone water (BPW). Generation times (g) and yields of Salmonella serovar Typhimurium were significantly affected by the type of peptone employed with yeast peptones generating yields of 7·04 × 109 CFU ml−1 and gelatin peptones producing 0·81 × 109 CFU ml−1. Medium sterilization was also found to have significant effects (P = 0·000) upon subsequent bacterial growth. Filter sterilization of BPW media produced lower generation times compared with those obtained after sterilization by autoclaving. Finally, it was observed that some peptones which produced good growth when inoculated with healthy organisms, showed relatively poor growth when inocula were sublethally injured by heating. Conclusions:  Variation in peptone as a constituent of BPW has a significant effect on growth and enumeration of bacteria. Significance and Impact of the Study:  Increased consideration with respect to culture media may significantly improve bacterial growth and experimental reproducibility.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Biosciences
Uncontrolled Keywords: growth; media; peptone; Salmonella; yield
Publisher: Wiley-Blackwell
ISSN: 1364-5072
Last Modified: 04 Jun 2017 02:06

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