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Models of ex vivo explant cultures: applications in bone research

Marino, Silvia, Staines, Katherine Ann, Brown, Genevieve, Howard-Jones, Rachel Anne and Adamczyk, Magdalena 2016. Models of ex vivo explant cultures: applications in bone research. BoneKEy Reports 5 , 818. 10.1038/bonekey.2016.49

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Ex vivo explant culture models are powerful tools in bone research. They allow investigation of bone and cartilage responses to specific stimuli in a controlled manner that closely mimics the in vivo processes. Because of limitations in obtaining healthy human bone samples the explant growth of animal tissue serves as a platform to study the complex physico-chemical properties of the bone. Moreover, these models enable preserving important cell–cell and cell–matrix interactions in order to better understand the behaviour of cells in their natural three-dimensional environment. Thus, the use of bone ex vivo explant cultures can frequently be of more physiological relevance than the use of two-dimensional primary cells grown in vitro. Here, we describe isolation and ex vivo growth of different animal bone explant models including metatarsals, femoral heads, calvaria, mandibular slices and trabecular cores. We also describe how these explants are utilised to study bone development, cartilage and bone metabolism, cancer-induced bone diseases, stem cell-driven bone repair and mechanoadaptation. These techniques can be directly used to understand mechanisms linked with bone physiology or bone-associated diseases.

Item Type: Article
Date Type: Published Online
Status: Published
Schools: Dentistry
Publisher: Nature Publishing Group
ISSN: 2047-6396
Date of First Compliant Deposit: 28 June 2016
Date of Acceptance: 4 May 2016
Last Modified: 25 May 2021 01:47

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