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Suppression of ClC-3 channel expression reduces migration of nasopharyngeal carcinoma cells

Mao, Jianwen, Chen, Lixen, Xu, Bin, Wang, Lijing, Li, Hongzhi, Guo, Jiao, Li, Weidong, Nie, Sihuai, Jacob, Timothy John Claud and Wang, Liwei 2008. Suppression of ClC-3 channel expression reduces migration of nasopharyngeal carcinoma cells. Biochemical Pharmacology 75 (9) , pp. 1706-1716. 10.1016/j.bcp.2008.01.008

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Recent studies suggest that chloride (Cl−) channels regulate tumor cell migration. In this report, we have used antisense oligonucleotides specific for ClC-3, the most likely molecular candidate for the volume-activated Cl− channel, to investigate the role of ClC-3 in the migration of nasopharyngeal carcinoma cells (CNE-2Z) in vitro. We found that suppression of ClC-3 expression inhibited the migration of CNE-2Z cells in a concentration-dependent manner. Whole-cell patch-clamp recordings and image analysis further demonstrated that ClC-3 suppression inhibited the volume-activated Cl− current (ICl,vol) and regulatory volume decrease (RVD) of CNE-2Z cells. The expression of ClC-3 positively correlated with cell migration, ICl,vol and RVD. These results strongly suggest that ClC-3 is a component or regulator of the volume-activated Cl− channel. ClC-3 may regulate CNE-2Z cell migration by modulating cell volume. ClC-3 may be a new target for cancer therapies.

Item Type: Article
Status: Published
Schools: Biosciences
Uncontrolled Keywords: ClC-3; Chloride channels; Antisense oligonucleotide; Cell migration; Cell volume
Publisher: Elsevier
ISSN: 0006-2952
Last Modified: 06 Jan 2022 02:23

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