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Volume-activated chloride channels contribute to cell-cycle-dependent regulation of HeLa cell migration

Mao, J., Chen, L., Xu, B., Wang, L., Wang, W., Li, M., Zheng, M., Li, H., Guo, J., Li, W., Jacob, Timothy John Claud and Wang, L. 2009. Volume-activated chloride channels contribute to cell-cycle-dependent regulation of HeLa cell migration. Biochemical Pharmacology 77 (2) , pp. 159-168. 10.1016/j.bcp.2008.10.009

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The activation of volume-activated chloride Cl− channels has been implicated to play important roles in modulating cell cycle and cell migration. The aim of this study was to determine whether volume-activated Cl− channels are involved in cell-cycle-dependent regulation of cell migration in HeLa cells. Using techniques including cell-cycle synchronization, transwell migration assays and the patch-clamp technique, we demonstrate in this study that both the expression of volume-activated chloride current (ICl,vol) and the potential of cell migration are cell-cycle-dependent; specifically, these events were high in G0/G1 phase, low in S phase, and medium in G2/M phase. Moreover, the mean density of ICl,vol was positively correlated to the rate of cell migration during cell-cycle progression. Additionally, endogenous suppression of ICl,vol by transfecting cells with ClC-3 antisense oligonucleotides arrested cells in S phase and slowed cell migration. Collectively, our results suggest that volume-activated Cl− channels contribute to the cell-cycle-dependent regulation of cell migration.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Biosciences
Publisher: Elsevier
ISSN: 0006-2952
Last Modified: 04 Jun 2017 02:07

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