P2X7 receptors are redistributed on human monocytes after pore formation in response to prolonged agonist exposure

Connon, Che John, Young, Robert David ORCID: https://orcid.org/0000-0002-8300-8002 and Kidd, Emma Jane ORCID: https://orcid.org/0000-0001-5507-1170 2003. P2X7 receptors are redistributed on human monocytes after pore formation in response to prolonged agonist exposure. Pharmacology 67 (3) , pp. 163-168. 10.1159/000067795

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Abstract

The P2X(7) cell surface receptor is responsible for adenosine triphosphate-dependent lysis of immune cells following the formation of non-selective membrane pores. This study examined changes in P2X(7) receptor distribution following agonist treatment. Human THP-1 monocytes were exposed to the agonist 2'-3'-O-(4-benzoyl-benzoyl) adenosine 5'-triphosphate for 1-40 min after which P2X(7) receptors were immunogold labelled. The number and distribution of P2X(7) receptors on the cell membrane were quantified using transmission electron microscopy. Increasing the time of agonist exposure resulted in clustering of P2X(7) receptors, a decrease in individual P2X(7) receptors and no change in the total number of P2X(7) receptors. These results suggest that pore formation does not involve further insertion or initial movement of receptors in the membrane but that P2X(7) receptors do cluster together after prolonged activation

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Biosciences Pharmacy Optometry and Vision Sciences
Uncontrolled Keywords:	P2X1 to P2X7 receptors ; Electron microscopy ; Clustering Cell surface ; Immunogold labelling
ISSN:	1423-0313
Last Modified:	17 Oct 2022 08:44
URI:	https://orca.cardiff.ac.uk/id/eprint/931

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