Identification of genes involved in the susceptibility of Serratia marcescens to polyquaternium-1

Codling, Caroline E., Jones, Brian V., Mahenthiralingam, Eshwar ORCID: https://orcid.org/0000-0001-9014-3790, Russell, A. Denver and Maillard, Jean-Yves ORCID: https://orcid.org/0000-0002-8617-9288 2004. Identification of genes involved in the susceptibility of Serratia marcescens to polyquaternium-1. Journal of Antimicrobial Chemotherapy 54 (2) , pp. 370-375. 10.1093/jac/dkh351

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Abstract

Objectives: Polyquaternium-1 (PQ-1) is a biocide used commercially in a contact lens disinfecting solution, ‘Opti-Free Express (Alcon) Multi-Purpose Disinfecting Solution’. The genetic basis for resistance of Serratia marcescens to PQ-1 was investigated using a random transposon-based mutagenesis approach. Methods: S. marcescens was subjected to random transposon mutagenesis using a mini-Tn5?Km2 transposon. Mutants with increased susceptibility to PQ-1 were selected and the disrupted genes were identified. Antibiotic susceptibility profiles were also determined for all of the mutants. Results: A wide range of genes were found to be disrupted in the mutants. The most common were genes associated with the cell membranes, or involved in biosynthesis and metabolism. Conclusions: This study shows that random transposon mutagenesis is an effective tool for the elucidation of mechanisms of action and resistance to biocides. The results support our previous findings that PQ-1 is active against the cytoplasmic membrane of S. marcescens.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Schools > Pharmacy
Uncontrolled Keywords:	S. marcescens biocides antibiotics transposon mutagenesis gene identification genetics
Publisher:	Oxford University Press
ISSN:	0305-7453
Last Modified:	17 Oct 2022 08:44
URI:	https://orca.cardiff.ac.uk/id/eprint/934

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