Aboklaish, Ali, Ahmed, S., McAllister, D., Cassell, G., Zheng, X. and Spiller, Owen Bradley  ORCID: https://orcid.org/0000-0002-9117-6911
2016.
Differential recognition of the Multiple Banded Antigen isoforms across Ureaplasma parvum and Ureaplasma urealyticum species by monoclonal antibodies.
Journal of Microbiological Methods
127
, pp. 13-19.
10.1016/j.mimet.2016.05.015
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Abstract
Two separate species of Ureaplasma have been identified that infect humans: Ureaplasma parvum and Ureaplasma urealyticum. Most notably, these bacteria lack a cell wall and are the leading infectious organism associated with infection-related induction of preterm birth. Fourteen separate representative prototype bacterial strains, called serovars, are largely differentiated by the sequence of repeating units in the C-terminus of the major surface protein: multiple-banded antigen (MBA). Monoclonal antibodies that recognise single or small groups of serovars have been previously reported, but these reagents remain sequestered in individual research laboratories. Here we characterise a panel of commercially available monoclonal antibodies raised against the MBA and describe the first monoclonal antibody that cross-reacts by immunoblot with all serovars of U. parvum and U. urealyticum species. We also describe a recombinant MBA expressed by Escherichia coli which facilitated further characterisation by immunoblot and demonstrate immunohistochemistry of paraffin-embedded antigens. Immunoblot reactivity was validated against well characterised previously published monoclonal antibodies and individual commercial antibodies were found to recognise all U. parvum strains, only serovars 3 and 14 or only serovars 1 and 6, or all strains belonging to U. parvum and U. urealyticum. MBA mass was highly variable between strains, consistent with variation in the number of C-terminal repeats between strains. Antibody characterisation will enable future investigations to correlate severity of pathogenicity to MBA isoform number or mass, in addition to development of antibody-based diagnostics that will detect infection by all Ureaplasma species or alternately be able to differentiate between U. parvum, U. urealyticum or mixed infections.
| Item Type: | Article |
|---|---|
| Date Type: | Publication |
| Status: | Published |
| Schools: | Schools > Medicine |
| Subjects: | R Medicine > RG Gynecology and obstetrics |
| Uncontrolled Keywords: | Ureaplasma parvum; Ureaplasma urealyticum; Multiple banded antigen; Recombinant protein |
| Publisher: | Elsevier |
| ISSN: | 0167-7012 |
| Date of First Compliant Deposit: | 24 October 2016 |
| Date of Acceptance: | 24 May 2016 |
| Last Modified: | 18 Nov 2024 19:45 |
| URI: | https://orca.cardiff.ac.uk/id/eprint/95440 |
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