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Generation of multipotent foregut stem cells from human pluripotent stem cells

Hannan, Nicholas R.F., Fordham, Robert P., Syed, Yasir A., Moignard, Victoria, Berry, Andrew, Bautista, Ruben, Hanley, Neil A., Jensen, Kim B. and Vallier, Ludovic 2013. Generation of multipotent foregut stem cells from human pluripotent stem cells. Stem Cell Reports 1 (4) , pp. 293-306. 10.1016/j.stemcr.2013.09.003

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Human pluripotent stem cells (hPSCs) could provide an infinite source of clinically relevant cells with potential applications in regenerative medicine. However, hPSC lines vary in their capacity to generate specialized cells, and the development of universal protocols for the production of tissue-specific cells remains a major challenge. Here, we have addressed this limitation for the endodermal lineage by developing a defined culture system to expand and differentiate human foregut stem cells (hFSCs) derived from hPSCs. hFSCs can self-renew while maintaining their capacity to differentiate into pancreatic and hepatic cells. Furthermore, near-homogenous populations of hFSCs can be obtained from hPSC lines which are normally refractory to endodermal differentiation. Therefore, hFSCs provide a unique approach to bypass variability between pluripotent lines in order to obtain a sustainable source of multipotent endoderm stem cells for basic studies and to produce a diversity of endodermal derivatives with a clinical value.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Biosciences
Publisher: Elsevier
ISSN: 2213-6711
Funders: Medical Research Council (MRC)
Date of First Compliant Deposit: 21 February 2017
Date of Acceptance: 1 October 2013
Last Modified: 04 Jun 2017 09:43

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