Community analysis of dental plaque and endotracheal tube biofilms from mechanically ventilated patients

Marino, Paola J., Wise, Matt P., Smith, Ann, Marchesi, Julian R. ORCID: https://orcid.org/0000-0002-7994-5239, Riggio, Marcello P, Lewis, Michael A O ORCID: https://orcid.org/0000-0003-1917-0651 and Williams, David W. ORCID: https://orcid.org/0000-0002-7351-5131 2017. Community analysis of dental plaque and endotracheal tube biofilms from mechanically ventilated patients. Journal of Critical Care 39 , pp. 149-155. 10.1016/j.jcrc.2017.02.020

Preview

PDF - Accepted Post-Print Version Download (1MB) | Preview

Abstract

Purpose: Mechanically ventilated patients are at risk of developing ventilator-associated pneumonia and it has been reported that dental plaque provides a reservoir of respiratory pathogens that may aspirate to the lungs and endotracheal tube (ETT) biofilms. For the first time, metataxonomics was used to simultaneously characterise the microbiome of dental plaque, ETTs and non-directed bronchial lavages (NBL) in mechanically ventilated patients to determine similarities in respective microbial communities and therefore likely associations. Material and Methods: Bacterial 16S rRNA gene sequences from 34 samples of dental plaque, NBLs and ETTs from 12 adult mechanically ventilated patients were analysed. Results: No significant differences in the microbial communities of these samples were evident. Detected bacteria were primarily oral species (e.g. Fusobacterium nucleatum, Streptococcus salivarius, Prevotella melaninogenica) with respiratory pathogens (Staphylococcus aureus, Pseudomonas aeruginosa, Streptococcus pneumoniae and Haemophilus influenzae) also in high abundance. Conclusion: The high similarity between the microbiomes of dental plaque, NBLs and ETTs suggests that the oral cavity is indeed an important site involved in microbial aspiration to the lower airway and ETT. As such, maintenance of good oral hygiene is likely to be highly important in limiting aspiration of bacterial in this vulnerable patient group.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Dentistry Biosciences
Subjects:	R Medicine > R Medicine (General) R Medicine > RK Dentistry
Uncontrolled Keywords:	Endotracheal tube biofilm, dental plaque, metataxonomics, mechanical ventilation
Publisher:	WB Saunders
ISSN:	0883-9441
Funders:	GSK-Oral and Dental Research Trust
Date of First Compliant Deposit:	28 February 2017
Date of Acceptance:	7 February 2017
Last Modified:	13 Nov 2024 23:15
URI:	https://orca.cardiff.ac.uk/id/eprint/98629

Citation Data

Cited 24 times in Scopus. View in Scopus. Powered By Scopus® Data

Actions (repository staff only)

Edit Item