Dual roles of protein tyrosine phosphatase kappa in coordinating angiogenesis induced by pro-angiogenic factors

Sun, Ping-Hui, Chen, Gang, Mason, Malcolm ORCID: https://orcid.org/0000-0003-1505-2869, Jiang, Wen ORCID: https://orcid.org/0000-0002-3283-1111 and Ye, Lin ORCID: https://orcid.org/0000-0002-0303-2409 2017. Dual roles of protein tyrosine phosphatase kappa in coordinating angiogenesis induced by pro-angiogenic factors. International Journal of Oncology 50 (4) , pp. 1127-1135. 10.3892/ijo.2017.3884

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Abstract

A potential role may be played by receptor-type protein tyrosine phosphatase kappa (PTPRK) in angiogenesis due to its critical function in coordinating intracellular signal transduction from various receptors reliant on tyrosine phosphorylation. In the present study, we investigated the involvement of PTPRK in the cellular functions of vascular endothelial cells (HECV) and its role in angiogenesis using in vitro assays and a PTPRK knockdown vascular endothelial cell model. PTPRK knockdown in HECV cells (HECVPTPRKkd) resulted in a decrease of cell proliferation and cell-matrix adhesion; however, increased cell spreading and motility were seen. Reduced focal adhesion kinase (FAK) and paxillin protein levels were seen in the PTPRK knockdown cells which may contribute to the inhibitory effect on adhesion. HECVPTPRKkd cells were more responsive to the treatment of fibroblast growth factor (FGF) in their migration compared with the untreated control and cells treated with VEGF. Moreover, elevated c-Src and Akt1 were seen in the PTPRK knockdown cells. The FGF-promoted cell migration was remarkably suppressed by an addition of PLCγ inhibitor compared with other small inhibitors. Knockdown of PTPRK suppressed the ability of HECV cells to form tubules and also impaired the tubule formation that was induced by FGF and conditioned medium of cancer cells. Taken together, it suggests that PTPRK plays dual roles in coordinating angiogenesis. It plays a positive role in cell proliferation, adhesion and tubule formation, but suppresses cell migration, in particular, the FGF-promoted migration. PTPRK bears potential to be targeted for the prevention of tumour associated angiogenesis.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Medicine
Subjects:	R Medicine > R Medicine (General)
Uncontrolled Keywords:	protein tyrosine phosphatase kappa, angiogenesis, endothelial cell, fibroblast growth factor, paxillin, PLCγ
Publisher:	Spandidos Publications
ISSN:	1019-6439
Funders:	Cancer Research Wales and Ser Cymru Welsh Life Science Research Network
Date of First Compliant Deposit:	8 March 2017
Date of Acceptance:	18 January 2017
Last Modified:	05 May 2023 08:44
URI:	https://orca.cardiff.ac.uk/id/eprint/98852

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