FGF-dependent generation of oligodendrocytes by a hedgehog-independent pathway

Chandran, Siddharthan, Kato, Hidemasa, Gerreli, Dianne, Compston, Alastair, Svendsen, Clive N. and Allen, Nicholas Denby ORCID: https://orcid.org/0000-0003-4009-186X 2003. FGF-dependent generation of oligodendrocytes by a hedgehog-independent pathway. Development Vol 13 (26) , pp. 6599-6609. 10.1242/dev.00871

Full text not available from this repository.

Abstract

During development, spinal cord oligodendrocyte precursors (OPCs) originate from the ventral, but not dorsal, neuroepithelium. Sonic hedgehog (SHH) has crucial effects on oligodendrocyte production in the ventral region of the spinal cord; however, less is known regarding SHH signalling and oligodendrocyte generation from neural stem cells (NSCs). We show that NSCs isolated from the dorsal spinal cord can generate oligodendrocytes following FGF2 treatment, a MAP kinase dependent phenomenon that is associated with induction of the obligate oligogenic gene Olig2. Cyclopamine, a potent inhibitor of hedgehog signalling, did not block the formation of oligodendrocytes from FGF2-treated neurosphere cultures. Furthermore, neurospheres generated from SHH null mice also produced oligodendrocytes, even in the presence of cyclopamine. These findings are compatible with the idea of a hedgehog independent pathway for oligodendrocyte generation from neural stem cells.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Biosciences Neuroscience and Mental Health Research Institute (NMHRI)
Uncontrolled Keywords:	Stem cell ; Oligodendrocyte ; FGF2 ; Spinal cord ; Sonic hedgehog ; OLIG2 ; NKX2.2
ISSN:	0950-1991
Last Modified:	17 Oct 2022 08:46
URI:	https://orca.cardiff.ac.uk/id/eprint/996

Citation Data

Cited 107 times in Scopus. View in Scopus. Powered By Scopus® Data

Actions (repository staff only)

Edit Item