Embryonic stem cell derived neural progenitors display temporal restriction to neural patterning

Bouhon, Isabelle A., Joannides, Alexis, Kato, Hidemasa, Chandran, Siddharthan and Allen, Nicholas Denby ORCID: https://orcid.org/0000-0003-4009-186X 2006. Embryonic stem cell derived neural progenitors display temporal restriction to neural patterning. Stem Cells 24 (8) , pp. 1908-1913. 10.1634/stemcells.2006-0031

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Abstract

Neural stem cells have considerable therapeutic potential because of their ability to generate defined neuronal cell types for use in drug screening studies or cell-based therapies for neurodegenerative diseases. In this study, we differentiate mouse embryonic stem cells to neural progenitors with an initial forebrain identity in a defined system that enables systematic manipulation to generate more caudal fates, including motoneurons. We demonstrate that the ability to pattern embryonic stem cell-derived neural progenitors is temporally restricted and show that the loss of responsiveness to morphogenetic cues correlates with constitutive expression of the basic helix-loop-helix transcription factors Olig2 and Mash1, epidermal growth factor receptor, and vimentin and parallels the onset of gliogenesis. We provide evidence for two temporal classes of embryonic stem cell-derived putative radial glia that coincide with a transition from neurogenesis to gliogenesis and a concomitant loss of regional identity.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Biosciences Neuroscience and Mental Health Research Institute (NMHRI)
Uncontrolled Keywords:	Embryonic stem cell ; Neural differentiation ; Patterning ; Temporal restriction ; Radial glia
ISSN:	1066-5099
Last Modified:	17 Oct 2022 08:46
URI:	https://orca.cardiff.ac.uk/id/eprint/999

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