Variation in human dental pulp stem cell ageing profiles reflect contrasting proliferative and regenerative capabilities

Alraies, Amr ORCID: https://orcid.org/0000-0003-1977-3487, Alaidaroos, Nadia Yousef A, Waddington, Rachel J. ORCID: https://orcid.org/0000-0001-5878-1434, Moseley, Ryan ORCID: https://orcid.org/0000-0002-2812-6735 and Sloan, Alastair J. ORCID: https://orcid.org/0000-0002-1791-0903 2017. Variation in human dental pulp stem cell ageing profiles reflect contrasting proliferative and regenerative capabilities. BMC Cell Biology 18 , 12. 10.1186/s12860-017-0128-x

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Abstract

Background Dental pulp stem cells (DPSCs) are increasingly being recognized as a viable cell source for regenerative medicine. Although significant variations in their ex vivo expansion are well-established, DPSC proliferative heterogeneity remains poorly understood, despite such characteristics influencing their regenerative and therapeutic potential. This study assessed clonal human DPSC regenerative potential and the impact of cellular senescence on these responses, to better understand DPSC functional behaviour. Results All DPSCs were negative for hTERT. Whilst one DPSC population reached >80 PDs before senescence, other populations only achieved <40 PDs, correlating with DPSCs with high proliferative capacities possessing longer telomeres (18.9 kb) than less proliferative populations (5–13 kb). High proliferative capacity DPSCs exhibited prolonged stem cell marker expression, but lacked CD271. Early-onset senescence, stem cell marker loss and positive CD271 expression in DPSCs with low proliferative capacities were associated with impaired osteogenic and chondrogenic differentiation, favouring adipogenesis. DPSCs with high proliferative capacities only demonstrated impaired differentiation following prolonged expansion (>60 PDs). Conclusions This study has identified that proliferative and regenerative heterogeneity is related to contrasting telomere lengths and CD271 expression between DPSC populations. These characteristics may ultimately be used to selectively screen and isolate high proliferative capacity/multi-potent DPSCs for regenerative medicine exploitation.

Item Type:	Article
Date Type:	Published Online
Status:	Published
Schools:	Dentistry
Subjects:	R Medicine > RK Dentistry
Uncontrolled Keywords:	Dental pulp – Stem cells – Cumulative population doublings – Telomeres – Cellular senescence – Differentiation – Multi-potency – CD271
Publisher:	BioMed Central
ISSN:	1471-2121
Date of First Compliant Deposit:	11 May 2017
Date of Acceptance:	20 January 2017
Last Modified:	10 May 2023 00:38
URI:	https://orca.cardiff.ac.uk/id/eprint/100523

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