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N-terminus oligomerization regulates the function of cardiac ryanodine receptors

Zissimopoulos, Spyros, Viero, C., Seidel, Monika, Cumbes, Bevan ORCID:, White, Judith, Cheung, I., Stewart, R., Jeyakumar, L., Fleischer, S., Mukherjee, Saptarshi, Thomas, Nia Lowri ORCID:, Williams, Alan John and Lai, Francis ORCID: 2013. N-terminus oligomerization regulates the function of cardiac ryanodine receptors. Journal of Cell Science 126 (21) , pp. 5042-5051. 10.1242/jcs.133538

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The ryanodine receptor (RyR) is an ion channel composed of four identical subunits mediating calcium efflux from the endo/sarcoplasmic reticulum of excitable and non-excitable cells. We present several lines of evidence indicating that the RyR2 N-terminus is capable of self-association. A combination of yeast two-hybrid screens, co-immunoprecipitation analysis, chemical crosslinking and gel filtration assays collectively demonstrate that a RyR2 N-terminal fragment possesses the intrinsic ability to oligomerize, enabling apparent tetramer formation. Interestingly, N-terminus tetramerization mediated by endogenous disulfide bond formation occurs in native RyR2, but notably not in RyR1. Disruption of N-terminal inter-subunit interactions within RyR2 results in dysregulation of channel activation at diastolic Ca2+ concentrations from ryanodine binding and single channel measurements. Our findings suggest that the N-terminus interactions mediating tetramer assembly are involved in RyR channel closure, identifying a crucial role for this structural association in the dynamic regulation of intracellular Ca2+ release.

Item Type: Article
Status: Published
Schools: Medicine
Subjects: R Medicine > R Medicine (General)
Publisher: Company of Biologists
ISSN: 0021-9533
Date of Acceptance: 30 July 2013
Last Modified: 22 Nov 2022 09:43

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