Impact of pigment epithelium-derived factor on colorectal cancer in vitro and in vivo

Harries, Rhiannon L., Owen, Sioned, Ruge, Fiona, Morgan, Meleri, Li, Jun, Zhang, Zhangtao, Harding, Keith G., Torkington, Jared, Jiang, Wen G. ORCID: https://orcid.org/0000-0002-3283-1111 and Cai, Jun 2018. Impact of pigment epithelium-derived factor on colorectal cancer in vitro and in vivo. Oncotarget 9 (27) , pp. 19192-19202. 10.18632/oncotarget.24953

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Abstract

Pigment epithelial derived factor (PEDF) is a secreted glycoprotein that is a non-inhibitory member of the serine protease inhibitor (serpin) family. PEDF exhibits multiple biological properties including neuroprotective, anti-angiogenic, and immune-modulating. Interestingly, PEDF exerts the inhibitory effects in cancers derived from certain tissues, including prostatic, ovarian, and pancreatic carcinomas. The current study aimed to elucidate its role in colorectal cancer development. PEDF expression in human colorectal cancer tissue was assessed using quantitative polymerase chain reaction (qPCR) and immunohistochemical staining (IHC). The effect of treatment with recombinant PEDF on cellular function was examined using in vitro functional assays. PEDF expression was downregulated in colorectal cancer cell tissue. Treatment with recombinant PEDF resulted in significant decreases in the rate of colorectal cancer cell migration and invasion and an increase in cellular adhesion in colorectal cancer cell lines examined. These results indicate that upregulation of PEDF expression may serve as a new strategy for further investigation of therapeutic relevance to the prevention of the metastatic spread of colorectal cancer.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Medicine
Publisher:	Impact Journals
ISSN:	1949-2553
Funders:	Royal College of Surgeons of England, Cancer Research Wales, Wales Life Science Network, Cardiff China Medical Scholarship
Date of First Compliant Deposit:	17 April 2018
Date of Acceptance:	14 March 2018
Last Modified:	15 Jul 2023 18:35
URI:	https://orca.cardiff.ac.uk/id/eprint/110753

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