Cobalt and Titanium nanoparticles influence on human osteoblast mitochondrial activity and biophysical properties of their cytoskeleton

Perni, Stefano, Yang, Lirong, Preedy, Emily Callard and Prokopovich, Polina ORCID: https://orcid.org/0000-0002-5700-9570 2018. Cobalt and Titanium nanoparticles influence on human osteoblast mitochondrial activity and biophysical properties of their cytoskeleton. Journal of Colloid and Interface Science 531 , pp. 410-420. 10.1016/j.jcis.2018.07.028

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Abstract

We investigated the biophysical effects (cell elasticity and spring constant) caused on Saos-2 human osteoblast-like cells by nanosized metal (Co and Ti) wear debris, as well as the adhesive characteristics of cells after exposure to the metal nanoparticles. Cell mitochondrial activity was investigated using the MTT assays; along with LDH assay, metal uptake, cell apoptosis and mineralisation output (alizarin red assay) of the cells. Osteoblasts mitochondrial activity was not affected by Ti nanoparticles at concentrations up to 1 mg/ml and by Cobalt nanoparticles at concentrations < 0.5 mg/ml; however elasticity and spring constant were significantly modified by the exposure to nanoparticles of these metals in agreement with the alteration of cell conformation (shape), as result of the exposure to simulated wear debris, demonstrated by fluorescence images after actin staining.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Pharmacy
Publisher:	Elsevier
ISSN:	0021-9797
Date of First Compliant Deposit:	30 July 2018
Date of Acceptance:	9 July 2018
Last Modified:	11 Nov 2024 02:30
URI:	https://orca.cardiff.ac.uk/id/eprint/113716

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