Neuronal basis of the slow (<1 Hz) oscillation in neurons of the nucleus reticularis thalami in vitro

Blethyn, Kate L., Hughes, Stuart Wynn, Tóth, Tibor Istvan, Cope, David W. and Crunelli, Vincenzo ORCID: https://orcid.org/0000-0001-7154-9752 2006. Neuronal basis of the slow (<1 Hz) oscillation in neurons of the nucleus reticularis thalami in vitro. Journal of Neuroscience 26 (9) , pp. 2474-2486. 10.1523/JNEUROSCI.3607-05.2006

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Abstract

During deep sleep and anesthesia, the EEG of humans and animals exhibits a distinctive slow (<1 Hz) rhythm. In inhibitory neurons of the nucleus reticularis thalami (NRT), this rhythm is reflected as a slow (<1 Hz) oscillation of the membrane potential comprising stereotypical, recurring "up" and "down" states. Here we show that reducing the leak current through the activation of group I metabotropic glutamate receptors (mGluRs) with either trans-ACPD [(+/–)-1-aminocyclopentane-trans-1,3-dicarboxylic acid] (50–100 µM) or DHPG [(S)-3,5-dihydroxyphenylglycine] (100 µM) instates an intrinsic slow oscillation in NRT neurons in vitro that is qualitatively equivalent to that observed in vivo. A slow oscillation could also be evoked by synaptically activating mGluRs on NRT neurons via the tetanic stimulation of corticothalamic fibers. Through a combination of experiments and computational modeling we show that the up state of the slow oscillation is predominantly generated by the "window" component of the T-type Ca2+ current, with an additional supportive role for a Ca2+-activated nonselective cation current. The slow oscillation is also fundamentally reliant on an Ih current and is extensively shaped by both Ca2+- and Na+-activated K+ currents. In combination with previous work in thalamocortical neurons, this study suggests that the thalamus plays an important and active role in shaping the slow (<1 Hz) rhythm during deep sleep.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Biosciences Neuroscience and Mental Health Research Institute (NMHRI)
Subjects:	R Medicine > RC Internal medicine > RC0321 Neuroscience. Biological psychiatry. Neuropsychiatry
Uncontrolled Keywords:	sleep; EEG; rhythm; thalamus; calcium current; T-type; mGluR; CAN current
Additional Information:	: “Copyright of all material published in The Journal of Neuroscience remains with the authors. The authors grant the Society for Neuroscience an exclusive license to publish their work for the first 6 months. After 6 months the work becomes available to the public to copy, distribute, or display under a Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported license.(http://creativecommons.org/licenses/)” See: http://www.jneurosci.org/site/misc/ifa_policies.xhtml
Publisher:	Society for Neuroscience
ISSN:	0022-3042
Last Modified:	30 May 2023 18:10
URI:	https://orca.cardiff.ac.uk/id/eprint/1161

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