LbL-assembled gentamicin delivery system for PMMA bone cements to prolong antimicrobial activity

Al Thaher, Yazan, Yang, Lirong, Jones, Steve A., Perni, Stefano and Prokopovich, Polina ORCID: https://orcid.org/0000-0002-5700-9570 2018. LbL-assembled gentamicin delivery system for PMMA bone cements to prolong antimicrobial activity. PLoS ONE 13 (12) , e0207753. 10.1371/journal.pone.0207753

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Abstract

Introduction Antibiotic-loaded poly(methyl methacrylate) bone cements (ALBCs) are widely used in total joint replacement (TJR), for local delivery of antibiotics to provide prophylaxis against prosthetic joint infections (PJI). One of the shortcomings of the current generation of ALBCs is that the antibiotic release profile is characterized by a burst over the first few hours followed by a sharp decrease in rate for the following several days (often below minimum inhibitory concentration (MIC)), and, finally, exhaustion (after, typically, ~ 20 d). This profile means that the ALBCs provide only short-term antimicrobial action against bacterial strains involved PJI. Rationale The purpose of the present study was to develop an improved antibiotic delivery system for an ALBC. This system involved using a layer-by-layer technique to load the antibiotic (gentamicin sulphate) (GEN) on silica nanoparticles, which are then blended with the powder of the cement. Then, the powder was mixed with the liquid of the cement (NP-GEN cement). For controls, two GEN-loaded brands were used (Cemex Genta and Palacos R+G). Gentamicin release and a host of other relevant properties were determined for all the cements studied. Results Compared to control cement specimens, improved GEN release, longer antimicrobial activity (against clinically-relevant bacterial strains), and comparable setting time, cytocompatibility, compressive strength (both prior to and after aging in PBS at 37 oC for 30 d), 4-point bend strength and modulus, fracture toughness, and PBS uptake. Conclusions NP-GEN cement may have a role in preventing or treating PJI.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Pharmacy
Additional Information:	This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) licence
Publisher:	Public Library of Science
ISSN:	1932-6203
Date of First Compliant Deposit:	14 December 2018
Date of Acceptance:	6 November 2018
Last Modified:	06 May 2023 22:14
URI:	https://orca.cardiff.ac.uk/id/eprint/117639

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