Formation of polarity convergences underlying shoot outgrowths

Abley, Katie, Sauret-Gueto, Susanna, Maree, Athanasius F. M. ORCID: https://orcid.org/0000-0003-2689-2484 and Coen, Enrico 2016. Formation of polarity convergences underlying shoot outgrowths. eLife 5 , e18165. 10.7554/eLife.18165

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Abstract

The development of outgrowths from plant shoots depends on formation of epidermal sites of cell polarity convergence with high intracellular auxin at their centre. A parsimonious model for generation of convergence sites is that cell polarity for the auxin transporter PIN1 orients up auxin gradients, as this spontaneously generates convergent alignments. Here we test predictions of this and other models for the patterns of auxin biosynthesis and import. Live imaging of outgrowths from kanadi1 kanadi2 Arabidopsis mutant leaves shows that they arise by formation of PIN1 convergence sites within a proximodistal polarity field. PIN1 polarities are oriented away from regions of high auxin biosynthesis enzyme expression, and towards regions of high auxin importer expression. Both expression patterns are required for normal outgrowth emergence, and may form part of a common module underlying shoot outgrowths. These findings are more consistent with models that spontaneously generate tandem rather than convergent alignments.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Biosciences
Additional Information:	This is an open access article under the terms of the CC-BY license.
Publisher:	eLife Sciences Publications
ISSN:	2050-084X
Date of First Compliant Deposit:	28 February 2019
Date of Acceptance:	28 June 2016
Last Modified:	05 May 2023 21:14
URI:	https://orca.cardiff.ac.uk/id/eprint/119482

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