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Visualizing the Spatiotemporal Dynamics of DNA Damage in Budding Yeast

Horigome, Chihiro, Dion, Vincent ORCID:, Seeber, Andrew, Gehlen, Lutz R. and Gasser, Susan M. 2015. Visualizing the Spatiotemporal Dynamics of DNA Damage in Budding Yeast. Stress reponses, Methods in Molecular Biology, New York: Springer New York, 77--96. (10.1007/978-1-4939-2522-3_6)

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Fluorescence microscopy has enabled the analysis of both the spatial distribution of DNA damage and its dynamics during the DNA damage response (DDR). Three microscopic techniques can be used to study the spatiotemporal dynamics of DNA damage. In the first part we describe how we determine the position of DNA double-strand breaks (DSBs) relative to the nuclear envelope. The second part describes how to quantify the co-localization of DNA DSBs with nuclear pore clusters, or other nuclear subcompartments. The final protocols describe methods for the quantification of locus mobility over time.

Item Type: Book Section
Status: Published
Schools: Medicine
Publisher: Springer New York
ISBN: 978-1-4939-2522-3
Last Modified: 25 Oct 2022 13:36

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