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Heterodyne dual-polarization epi-detected CARS microscopy for chemical and topographic imaging of interfaces

Langbein, Wolfgang ORCID:, Harlow, Dafydd S., Regan, David ORCID:, Pope, Iestyn ORCID: and Borri, Paola ORCID: 2019. Heterodyne dual-polarization epi-detected CARS microscopy for chemical and topographic imaging of interfaces. Presented at: SPIE BIOS, San Francisco, California, US, 2-7 Feb 2019. Label-free Biomedical Imaging and Sensing (LBIS) 2019. Proceedings of SPIE. , vol.10890 Bellingham, Washington: Society of Photo-optical Instrumentation Engineers (SPIE), 10.1117/12.2507636

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We present a label-free vibrational microscopy technique recently developed by us, which offers backgroundfree chemically-specific image contrast, shot-noise limited detection, and phase sensitivity enabling topographic imaging of interfaces. The technique features interferometric heterodyne detection of coherent anti-Stokes Raman scattering (CARS) in epi-geometry, as well as multi-modal acquisition of stimulated Raman scattering and forward-emitted CARS intensity in the same instrument. As an important biologically-relevant application, epi-detected heterodyne CARS imaging of individual lipid bilayers is demonstrated. We show that we can resolve a single lipid bilayer, distinct from a double bilayer, and measure the phase of its susceptibility, which provides information about the topography of the bilayer with nanometer resolution. As an additional application example, we show imaging of silicon oil droplets surrounded by an aqueous environment at the glass-water interface, where three different signal generation pathways are distinguished. Our epi-detected heterodyne CARS microscope setup thus paves the way to exciting new experiments pushing the sensitivity and resolution limits of vibrational microscopy to the nanoscale.

Item Type: Conference or Workshop Item (Paper)
Date Type: Publication
Status: Published
Schools: Physics and Astronomy
Publisher: Society of Photo-optical Instrumentation Engineers (SPIE)
ISBN: 9781510624238
ISSN: 0277-786X
Date of First Compliant Deposit: 18 March 2019
Last Modified: 05 Jan 2024 08:04

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