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Quantitative high-throughput optical sizing of individual colloidal nanoparticles by wide-field imaging extinction microscopy

Payne, Lukas, Zilli, Attilio, Wang, Yisu, Langbein, Wolfgang ORCID: and Borri, Paola ORCID: 2019. Quantitative high-throughput optical sizing of individual colloidal nanoparticles by wide-field imaging extinction microscopy. Presented at: SPIE BIOS, San Francisco, CA, USA, 2-7 February 2019. Proceedings Volume 10892, Colloidal Nanoparticles for Biomedical Applications XIV. , vol.108920 Society of Photo-Optical Instrumentation Engineers (SPIE), p. 17. 10.1117/12.2507632

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We present a wide-field imaging technique recently developed by us to measure quantitatively the optical extinction cross section σext of individual nanoparticles. The technique is simple, high speed, and enables the simultaneous acquisition of hundreds of nanoparticles in the wide-field image for statistical analysis, with a sensitivity corresponding to the detection of a single gold nanoparticle down to 2nm diameter. Notably, the method is applicable to any nanoparticle (dielectric, semiconducting, metallic), and can be easily and cost-effectively implemented on a conventional wide-field microscope. Of specific significance for accurate quantification, we show that σext depends on the numerical aperture of the microscope illumination due to the oblique incidence, even for spherical particles in an isotropic environment. This "long shadow" effect needs to be taken into account when comparing σext to theoretical values calculated under plane wave illumination at normal incidence. Owing to the accurate experimental quantification of σext, one can then use it to determine the nanoparticle size, as demonstrated here on gold nanoparticles of 30nm nominal diameter. This technique thus has the potential to become a simple and cost-effective new tool for accurate size characterization of single small nanoparticles, complementing time consuming and expensive methods such as electron microscopy.

Item Type: Conference or Workshop Item (Paper)
Date Type: Publication
Status: Published
Schools: Biosciences
Physics and Astronomy
Publisher: Society of Photo-Optical Instrumentation Engineers (SPIE)
Date of First Compliant Deposit: 14 March 2019
Date of Acceptance: 7 March 2019
Last Modified: 05 Jan 2024 06:46

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