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Imaging and tracking single plasmonic nanoparticles in 3D background-free with four-wave mixing interferometry

Borri, Paola ORCID:, Giannakopoulou, Naya, Zoriniants, George, Pope, Iestyn ORCID:, Masia, Francesco ORCID:, Watson, Peter ORCID: and Langbein, Wolfgang ORCID: 2019. Imaging and tracking single plasmonic nanoparticles in 3D background-free with four-wave mixing interferometry. Presented at: SPIE BIOS, San Francisco, CA, USA, 2-7 February 2019. Proceedings Volume 10894, Plasmonics in Biology and Medicine XVI. , vol.108940 Society of Photo-Optical Instrumentation Engineers (SPIE), p. 34. 10.1117/12.2507618

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We present a four-wave mixing interferometry technique recently developed by us, whereby single non-fluorescing gold nanoparticles are imaged background-free even inside highly heterogeneous cellular environments, owing to their specific nonlinear plasmonic response. The set-up enables correlative four-wave mixing/confocal fluorescence imaging, opening the prospect to study the fate of nanoparticle-biomolecule-fluorophore conjugates and their integrity inside cells. Beyond imaging, the technique features the possibility to track single particles with nanometric position localization precision in 3D from rapid single-point measurements at 1 ms acquisition time, by exploiting the optical vortex field pattern in the focal plane of a high numerical aperture objective lens. These measurements are also uniquely sensitive to the particle in-plane asymmetry and orientation. The localization precision in plane is found to be consistent with the photon shot-noise, while axially it is limited to about 3nm by the nano-positioning sample stage, with an estimated photon shot-noise limit of below 1 nm. As a proof-of- principle, the axial localization is exploited to track single gold nanoparticles of 25nm radius while diffusing across aqueous pockets in a dense agarose gel, mimicking a relevant biological environment.

Item Type: Conference or Workshop Item (Paper)
Date Type: Publication
Status: Published
Schools: Physics and Astronomy
Publisher: Society of Photo-Optical Instrumentation Engineers (SPIE)
Date of First Compliant Deposit: 14 March 2019
Date of Acceptance: 7 March 2019
Last Modified: 14 Sep 2023 01:05

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