On-demand electrical switching of antibody-antigen binding on surfaces

Gomes, Barbara Santos, Cantini, Eleonora, Tommasone, Stefano, Gibson, Joshua S., Wang, Xingyong, Zhu, Qiang, Ma, Jing, McGettrick, James D., Watson, Trystan M., Preece, Jon A., Kirkman-Brown, Jackson C., Publicover, Stephen J. and Mendes, Paula M. 2018. On-demand electrical switching of antibody-antigen binding on surfaces. ACS Applied Bio Materials 1 (3) , pp. 738-747. 10.1021/acsabm.8b00201

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Abstract

The development of stimuli-responsive interfaces between synthetic materials and biological systems is providing the unprecedented ability to modulate biomolecular interactions for a diverse range of biotechnological and biomedical applications. Antibody–antigen binding interactions are at the heart of many biosensing platforms, but no attempts have been made yet to control antibody–antigen binding in an on-demand fashion. Herein, a molecular surface was designed and developed that utilizes an electric potential to drive a conformational change in surface bound peptide moiety, to give on-demand control over antigen–antibody interactions on sensor chips. The molecularly engineered surfaces allow for propagation of conformational changes from the molecular switching unit to a distal progesterone antigen, resulting in promotion (ON state) or inhibition (OFF state) of progesterone antibody binding. The approach presented here can be generally applicable to other antigen–antibody systems and meets the technological needs for in situ long-term assessment of biological processes and disease monitoring on-demand.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Biosciences
Publisher:	American Chemical Society
ISSN:	2576-6422
Date of First Compliant Deposit:	15 April 2019
Date of Acceptance:	24 July 2018
Last Modified:	06 May 2023 01:04
URI:	https://orca.cardiff.ac.uk/id/eprint/121138

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