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Proteasomal degradation of BRAHMA promotes Boron tolerance in Arabidopsis

Sakamoto, Takuya, Tsujimoto-Inui, Yayoi, Sotta, Naoyuki, Hirakawa, Takeshi, Matsunaga, Tomoko, Fukao, Yoichiro, Matsunaga, Sachihiro and Fujiwara, Toru 2018. Proteasomal degradation of BRAHMA promotes Boron tolerance in Arabidopsis. Nature Communications 9 , pp. 1-16. 10.1038/s41467-018-07393-6

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Abstract

High levels of boron (B) induce DNA double-strand breaks (DSBs) in eukaryotes, including plants. Here we show a molecular pathway of high B-induced DSBs by characterizing Arabidopsis thaliana hypersensitive to excess boron mutants. Molecular analysis of the mutants revealed that degradation of a SWItch/Sucrose Non-Fermentable subunit, BRAHMA (BRM), by a 26S proteasome (26SP) with specific subunits is a key process for ameliorating high-B-induced DSBs. We also found that high-B treatment induces histone hyperacetylation, which increases susceptibility to DSBs. BRM binds to acetylated histone residues and opens chromatin. Accordingly, we propose that the 26SP limits chromatin opening by BRM in conjunction with histone hyperacetylation to maintain chromatin stability and avoid DSB formation under high-B conditions. Interestingly, a positive correlation between the extent of histone acetylation and DSB formation is evident in human cultured cells, suggesting that the mechanism of DSB induction is also valid in animals.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Biosciences
Publisher: Nature Publishing Group
ISSN: 2041-1723
Date of First Compliant Deposit: 14 May 2019
Date of Acceptance: 5 September 2018
Last Modified: 10 Nov 2021 02:23
URI: https://orca.cardiff.ac.uk/id/eprint/122317

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