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Preparing thin cross sections of Arabidopsis roots without embedding

Sotta, N. and Fujiwara, T. 2018. Preparing thin cross sections of Arabidopsis roots without embedding. Biotechniques 63 (6) , pp. 281-283. 10.2144/000114621

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Here, we describe a method for obtaining thin cross sections of Arabidopsis thaliana roots without fixation and embedding. Roots were grown in pinholes made in a solidified growth medium, and cross sections were prepared without pretreatment. Using this method, we detected unique distributions of two polar-localized proteins—green fluorescent protein (GFP)-tagged BOR1 and NIP5;1—with less sample preparation time than conventional methods. This method is simple, rapid, and yields high-quality cross-section images that are free from artifacts commonly associated with embedding or the sample preparation procedures used in many conventional methods.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Biosciences
Publisher: Future Science
ISSN: 0736-6205
Date of Acceptance: 1 November 2017
Last Modified: 10 Nov 2021 02:23

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