Cardiff University | Prifysgol Caerdydd ORCA
Online Research @ Cardiff 
WelshClear Cookie - decide language by browser settings

Emergence of plasmid-mediated high-level tigecycline resistance genes in animals and humans

He, Tao, Wang, Ran, Liu, Dejun, Walsh, Timothy R., Zhang, Rong, Lv, Yuan, Ke, Yuebin, Ji, Quanjiang, Wei, Ruicheng, Liu, Zhihai, Shen, Yingbo, Wang, Gang, Sun, Lichang, Lei, Lei, Lv, Ziquan, Li, Yun, Pang, Maoda, Wang, Liyuan, Sun, Qiaoling, Fu, Yulin, Song, Huangwei, Hao, Yuxin, Shen, Zhangqi, Wang, Shaolin, Chen, Gongxiang, Wu, Congming, Shen, Jianzhong and Wang, Yang 2019. Emergence of plasmid-mediated high-level tigecycline resistance genes in animals and humans. Nature Microbiology 10.1038/s41564-019-0445-2

Full text not available from this repository.


Tigecycline is a last-resort antibiotic that is used to treat severe infections caused by extensively drug-resistant bacteria. tet(X) has been shown to encode a flavin-dependent monooxygenase that modifies tigecycline1,2. Here, we report two unique mobile tigecycline-resistance genes, tet(X3) and tet(X4), in numerous Enterobacteriaceae and Acinetobacter that were isolated from animals, meat for consumption and humans. Tet(X3) and Tet(X4) inactivate all tetracyclines, including tigecycline and the newly FDA-approved eravacycline and omadacycline. Both tet(X3) and tet(X4) increase (by 64–128-fold) the tigecycline minimal inhibitory concentration values for Escherichia coli, Klebsiella pneumoniae and Acinetobacter baumannii. In addition, both Tet(X3) (A. baumannii) and Tet(X4) (E. coli) significantly compromise tigecycline in in vivo infection models. Both tet(X3) and tet(X4) are adjacent to insertion sequence ISVsa3 on their respective conjugative plasmids and confer a mild fitness cost (relative fitness of >0.704). Database mining and retrospective screening analyses confirm that tet(X3) and tet(X4) are globally present in clinical bacteria—even in the same bacteria as blaNDM-1, resulting in resistance to both tigecycline and carbapenems. Our findings suggest that both the surveillance of tet(X) variants in clinical and animal sectors and the use of tetracyclines in food production require urgent global attention.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Medicine
Publisher: Nature Research
ISSN: 2058-5276
Date of Acceptance: 2 May 2019
Last Modified: 17 Jun 2019 09:15

Citation Data

Cited 185 times in Scopus. View in Scopus. Powered By Scopus® Data

Actions (repository staff only)

Edit Item Edit Item