Cardiff University | Prifysgol Caerdydd ORCA
Online Research @ Cardiff 
WelshClear Cookie - decide language by browser settings

Molecular codes and in vitro generation of hypocretin and melanin concentrating hormone neurons

Seifinejad, Ali, Li, Sha, Mikhail, Cyril, Vassalli, Anne, Pradervand, Sylvain, Arribat, Yoan, Pezeshgi Modarres, Hassan, Allen, Bridget, John, Rosalind M. ORCID:, Amati, Francesca and Tafti, Mehdi 2019. Molecular codes and in vitro generation of hypocretin and melanin concentrating hormone neurons. Proceedings of the National Academy of Sciences 116 (34) , pp. 17061-17070. 10.1073/pnas.1902148116

[thumbnail of 1902148116.full.pdf] PDF - Published Version
Available under License Creative Commons Attribution Non-commercial No Derivatives.

Download (2MB)


Hypocretin (HCRT) and melanin concentrating hormone are brain neuropeptides involved in multiple functions, including sleep and metabolism. Loss of HCRT causes the sleep disorder narcolepsy. To understand how these neuropeptides are produced and contribute to diverse functions in health and disease, we purified their cells from mouse embryonic brains and established their molecular machinery. We discovered that partial removal of PEG3 (a transcription factor) in mice significantly reduces the number of HCRT and melanin concentrating hormone neurons, and its down-regulation in zebrafish completely abolishes their expression. We used our molecular data to produce these neurons in vitro from mouse fibroblasts, a technique that can be applied to cells from narcolepsy patients to generate an in vitro cell-based model.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Biosciences
Additional Information: This open access article is distributed under Creative Commons Attribution-NonCommercial-NoDerivatives License 4.0 (CC BY-NC-ND).
Publisher: National Academy of Sciences
ISSN: 0027-8424
Date of First Compliant Deposit: 6 August 2019
Date of Acceptance: 7 July 2019
Last Modified: 06 May 2023 00:21

Citation Data

Cited 15 times in Scopus. View in Scopus. Powered By Scopus® Data

Actions (repository staff only)

Edit Item Edit Item


Downloads per month over past year

View more statistics