Increase in lysophosphatidate acyltransferase activity in oilseed rape (Brassica napus) increases seed triacylglycerol content despite its low intrinsic flux control coefficient

Woodfield, Helen K. ORCID: https://orcid.org/0000-0002-0839-6105, Fenyk, Stepan, Wallington, Emma, Bates, Ruth E., Brown, Alexander, Guschina, Irina A., Marillia, Elizabeth-France, Taylor, David C., Fell, David, Harwood, John L. ORCID: https://orcid.org/0000-0003-2377-2612 and Fawcett, Tony 2019. Increase in lysophosphatidate acyltransferase activity in oilseed rape (Brassica napus) increases seed triacylglycerol content despite its low intrinsic flux control coefficient. New Phytologist 224 (2) , pp. 700-711. 10.1111/nph.16100

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Abstract

Lysophosphatidate acyltransferase (LPAAT ) catalyses the second step of the Kennedy pathway for triacylglycerol (TAG ) synthesis. In this study we expressed Trapaeolum majus LPAAT in Brassica napus (B. napus ) cv 12075 to evaluate the effects on lipid synthesis and estimate the flux control coefficient for LPAAT . We estimated the flux control coefficient of LPAAT in a whole plant context by deriving a relationship between it and overall lipid accumulation, given that this process is a exponential. Increasing LPAAT activity resulted in greater TAG accumulation in seeds of between 25% and 29%; altered fatty acid distributions in seed lipids (particularly those of the Kennedy pathway); and a redistribution of label from 14C‐glycerol between phosphoglycerides. Greater LPAAT activity in seeds led to an increase in TAG content despite its low intrinsic flux control coefficient on account of the exponential nature of lipid accumulation that amplifies the effect of the small flux increment achieved by increasing its activity. We have also developed a novel application of metabolic control analysis likely to have broad application as it determines the in planta flux control that a single component has upon accumulation of storage products.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Biosciences
Additional Information:	This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
Publisher:	Wiley
ISSN:	0028-646X
Date of First Compliant Deposit:	12 August 2019
Date of Acceptance:	23 July 2019
Last Modified:	04 May 2023 18:32
URI:	https://orca.cardiff.ac.uk/id/eprint/124883

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