Lopez-Amoros, R, Mason, DJ and Lloyd, D ORCID: https://orcid.org/0000-0002-5656-0571 2000. Use of two oxonols and a fluorescent tetrazolium dye to monitor starvation of Escherichia coli in seawater by flow cytometry. Journal of Microbiological Methods 22 (2) , 165--176. 10.1016/0167-7012(94)00073-G |
Official URL: https://doi.org/10.1016/0167-7012(94)00073-G
Abstract
Flow cytometry was used to study starvation of Escherichia coli in artificial seawater using two membrane potential sensitive dyes and redox dye. The oxonols, DiBAC4(3) and oxonol VI, enabled assessment of energization states. Parallel use of these two dyes suggests that oxonol VI is the more useful for detection of membrane depolarization. The NADPH reducibility of 5-cyano-2,3-ditolyl tetrazolium chloride can be used to estimate loss of membrane integrity during survival in seawater.
Item Type: | Article |
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Date Type: | Published Online |
Status: | Published |
Schools: | Biosciences |
Publisher: | Elsevier |
ISSN: | 0167-7012 |
Date of Acceptance: | 10 October 1994 |
Last Modified: | 26 Oct 2022 08:35 |
URI: | https://orca.cardiff.ac.uk/id/eprint/127842 |
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